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Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging
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April 14, 2011
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An experimental mouse model of bone metastasis was established following intracardiac delivery of luciferase expressing mammary tumor cells. Tumor development and resulted osteolytic lesion were monitored longitudinally with bioluminescence and micro CT imaging.
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Tumor MetastasesOsteolytic LesionsBioluminescence ImagingMicro CT ImagingMDA-MB-231-luc-D3H2LN CellsNu/Nu MiceIntracardiac DeliverySystemic MetastasesIVIS SpectrumDLIT ReconstructionTumor SignalTibiaFemurMicro CT SystemQuantum FXX-ray DoseRadiation DoseLD50Mouse Imaging Shuttle DeviceAnimal PositioningCo-registrationLiving Image 4.1 Software
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Lim, E., Modi, K., Christensen, A., Meganck, J., Oldfield, S., Zhang, N. Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging. J. Vis. Exp. (50), e2775, doi:10.3791/2775 (2011).
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