This content is Free Access.
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
64,174 Views
•
09:56 min
•
May 23, 2011
DOI:
Özet
Automatically generated
We demonstrate application of the fluorescence indicator, TMRM, in cortical neurons to determine the relative changes in TMRM fluorescence intensity before and after application of a specific stimulus. We also show application of the fluorescence probe H2DCF-DA to assess the relative level of reactive oxygen species in cortical neurons.
Explore More Videos
Mitochondrial Membrane PotentialReactive Oxygen SpeciesLive Rat Cortical NeuronsRespiratory ChainATP GenerationOxidative StressEnvironmental ToxinsOxidative PhosphorylationMitochondrial DysfunctionFluorescent ProbesRhodamine 123TMRMTMREJC-1Fluorescence IndicatorsDihydroethidiumDihydrorhodamine 123H2DCF-DAFluorescence Intensity
Read Article
Cite this Article
Joshi, D. C., Bakowska, J. C. Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons. J. Vis. Exp. (51), e2704, doi:10.3791/2704 (2011).
Copy