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Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons

63,970 Views

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09:56 min

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May 23, 2011

DOI:

10.3791/2704-v

DOI:
10.3791/2704-v

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09:56 min
May 23, 2011

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10 Views
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1Department of Molecular Pharmacology and Experimental Therapeutics,Loyola University Chicago

Tags

Mitochondrial Membrane PotentialReactive Oxygen SpeciesLive Rat Cortical NeuronsRespiratory ChainATP GenerationOxidative StressEnvironmental ToxinsOxidative PhosphorylationMitochondrial DysfunctionFluorescent ProbesRhodamine 123TMRMTMREJC-1Fluorescence IndicatorsDihydroethidiumDihydrorhodamine 123H2DCF-DAFluorescence Intensity

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Joshi, D. C., Bakowska, J. C. Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons. J. Vis. Exp. (51), e2704, doi:10.3791/2704 (2011).

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