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Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
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June 17, 2011
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We describe a protocol that allows imaging of mitochondria in living neurons via fluorescence microscopy over long durations. Imaging over extended periods is accomplished through lentivirus-mediated expression of a mitochondrially targeted fluorescent protein and use of an inexpensive stage-top incubator that was designed and built in our laboratory.
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NeuromodulationMitochondrial TransportLive ImagingHippocampal NeuronsExtended DurationsVital DyesFluorescent ProteinsCytoskeletal ComponentsOrganellesDynamic Fluorescence MicroscopyRhodamine 123Mitochondrially Targeted EYFPPrimary NeuronsCulture ConditionsTemperatureHumidityPHFluorescent SignalSignal IntensityImage AnalysisExposure TimesPhotobleachingPhototoxicity
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Edelman, D. B., Owens, G. C., Chen, S. Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations. J. Vis. Exp. (52), e2599, doi:10.3791/2599 (2011).
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