/
/
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
This content is Free Access.
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
15,656 Views
•
04:50 min
•
June 17, 2011
DOI:
Summary
Automatically generated
We describe a protocol that allows imaging of mitochondria in living neurons via fluorescence microscopy over long durations. Imaging over extended periods is accomplished through lentivirus-mediated expression of a mitochondrially targeted fluorescent protein and use of an inexpensive stage-top incubator that was designed and built in our laboratory.
Explore More Videos
NeuromodulationMitochondrial TransportLive ImagingHippocampal NeuronsExtended DurationsVital DyesFluorescent ProteinsCytoskeletal ComponentsOrganellesDynamic Fluorescence MicroscopyRhodamine 123Mitochondrially Targeted EYFPPrimary NeuronsCulture ConditionsTemperatureHumidityPHFluorescent SignalSignal IntensityImage AnalysisExposure TimesPhotobleachingPhototoxicity
Read Article
Cite this Article
Edelman, D. B., Owens, G. C., Chen, S. Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations. J. Vis. Exp. (52), e2599, doi:10.3791/2599 (2011).
Copy