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Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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February 23, 2011
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Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.
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AssayingSurface ExpressionChemosensory ReceptorsHeterologous CellsOlfactionG Protein Coupled Receptors (GPCRs)Odorant MoleculesIntracellular SignalG Protein GαolfCombinatorial RuleBiochemical StudiesLigand Binding StudiesHeterologous CellsReceptor Transporting Protein (RTP)Olfactory Sensory NeuronsChaperonesTransfectionBiochemical AssaysCell LineLive Cell Staining
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Dey, S., Zhan, S., Matsunami, H. Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells. J. Vis. Exp. (48), e2405, doi:10.3791/2405 (2011).
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