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Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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04:55 min
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February 23, 2011
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Summary
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Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.
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Dey, S., Zhan, S., Matsunami, H. Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells. J. Vis. Exp. (48), e2405, doi:10.3791/2405 (2011).
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