Obtaining Horizontal Mouse Hippocampal Brain Slices

Take the mouse brain hemispheres in a high-sucrose solution. Secure the hemispheres with their ventral side facing up on a vibratome specimen plate using an adhesive.

Transfer the specimen plate into the slicing chamber.

Add a semi-frozen high-sucrose solution to maintain the tissue's physiological conditions and solidify the adhesive.

Supply a carbon dioxide-oxygen mixture to maintain tissue oxygenation.

Using set parameters, obtain the initial slices and remove them. Further, obtain horizontal hippocampal slices.

Carefully transfer these brain slices into a chamber containing ACSF for tissue recovery.

The horizontal hippocampal slices are ready for further experimentation.