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Obtaining Horizontal Mouse Hippocampal Brain Slices
Transcrição
Take the mouse brain hemispheres in a high-sucrose solution. Secure the hemispheres with their ventral side facing up on a vibratome specimen plate using an adhesive.
Transfer the specimen plate into the slicing chamber.
Add a semi-frozen high-sucrose solution to maintain the tissue's physiological conditions and solidify the adhesive.
Supply a carbon dioxide-oxygen mixture to maintain tissue oxygenation.
Using set parameters, obtain the initial slices and remove them. Further, obtain horizontal hippocampal slices.
Carefully transfer these brain slices into a chamber containing ACSF for tissue recovery.
The horizontal hippocampal slices are ready for further experimentation.
