Dergi
/
Nörobilim
/
Strategies for Study of Neuroprotection from Cold-preconditioning
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Strategies for Study of Neuroprotection from Cold-preconditioning

Strategies for Study of Neuroprotection from Cold-preconditioning

DOI:
10.3791/2192-v

•

16:27 min

•

September 02, 2010

•

, ,
1Department of Neurology,The University of Chicago Medical Center

Bölümler

  • 00:00Başlık
  • 00:50Introduction
  • 01:46Preparation and Maintenance of Hippocampal Slice Cultures
  • 05:34Pre-screen Vitality of Slice Cultures
  • 06:47Cold-preconditioning
  • 07:36Excitotoxic Injury
  • 10:43Representative Imaging Results
  • 14:24Discussion
  • 15:36Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We seek to define the neural immune signaling responsible for cold-preconditioning as means to identify novel targets for therapeutics development to protect brain before injury onset. We present strategies for such work that require biological systems, experimental manipulations plus technical capacities that are highly reproducible and sensitive.

Etiketler

NeuroprotectionCold-preconditioningNeurological InjuryAnesthesiaSurgical ProceduresPreconditioning TreatmentsNeural Immune SignalingTherapeutics DevelopmentPro-inflammatory Mediator SignalingPhysiological Conditioning HormesisIrritative StimuliAdaptationImmune SignalingHippocampal Slice CulturesIn Vitro ModelMulti-synaptic Neural NetworksMacrogliaMicroglia

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Laser Capture Microdissection of Drosophila Peripheral Neurons

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia

Read Article