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神经科学
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Strategies for Study of Neuroprotection from Cold-preconditioning
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Strategies for Study of Neuroprotection from Cold-preconditioning

Strategies for Study of Neuroprotection from Cold-preconditioning

DOI:
10.3791/2192-v

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16:27 min

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September 02, 2010

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, ,
1Department of Neurology,The University of Chicago Medical Center

Chapters

  • 00:00Title
  • 00:50Introduction
  • 01:46Preparation and Maintenance of Hippocampal Slice Cultures
  • 05:34Pre-screen Vitality of Slice Cultures
  • 06:47Cold-preconditioning
  • 07:36Excitotoxic Injury
  • 10:43Representative Imaging Results
  • 14:24Discussion
  • 15:36Conclusion

Summary

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We seek to define the neural immune signaling responsible for cold-preconditioning as means to identify novel targets for therapeutics development to protect brain before injury onset. We present strategies for such work that require biological systems, experimental manipulations plus technical capacities that are highly reproducible and sensitive.

Tags

NeuroprotectionCold-preconditioningNeurological InjuryAnesthesiaSurgical ProceduresPreconditioning TreatmentsNeural Immune SignalingTherapeutics DevelopmentPro-inflammatory Mediator SignalingPhysiological Conditioning HormesisIrritative StimuliAdaptationImmune SignalingHippocampal Slice CulturesIn Vitro ModelMulti-synaptic Neural NetworksMacrogliaMicroglia

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