/
/
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
11,648 Views
•
11:37 min
•
July 08, 2010
DOI:
Özet
Automatically generated
This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.
Explore More Videos
Calpain ActivityFlow CytometryFixed CellsLiving CellsCalcium-sensitive ProteasesPhysiological ProcessesSignalingCytoskeletal RemodelingGene Expression RegulationApoptosisCell Cycle ProgressionPathologiesMuscular DystrophiesCancerDiabetesAlzheimer’s DiseaseMultiple SclerosisCalpain RegulationMRNA LevelsProtein LevelsGene Expression TechniquesFluorescent Substrate BOC-LM-CMACCalpain Activity MeasurementMurine 32Dkit Leukemia CellsSplenocyte Population
Read Article
Cite this Article
Farr, C., Berger, S. Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry. J. Vis. Exp. (41), e2050, doi:10.3791/2050 (2010).
Copy