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Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

DOI:
10.3791/2050-v

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11:37 min

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July 08, 2010

•

,
1Immunology,University of Toronto, 2Arthritis and Immune Disorder Research Centre,University Health Network (UHN)

Kapitel

  • 00:06Titel
  • 00:48Introduction
  • 01:15Reagent Preparation
  • 01:56Cell Preparation
  • 03:11Calpain Assay in Fixed Cells
  • 04:48Data Acquisition (Fixed Cells)
  • 06:55Calpain Assay in Live Cells
  • 08:22Calpain Assay in Mixed Cells
  • 10:05Analysis and Representative Results
  • 11:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.

Tags

Calpain ActivityFlow CytometryFixed CellsLiving CellsCalcium-sensitive ProteasesPhysiological ProcessesSignalingCytoskeletal RemodelingGene Expression RegulationApoptosisCell Cycle ProgressionPathologiesMuscular DystrophiesCancerDiabetesAlzheimer’s DiseaseMultiple SclerosisCalpain RegulationMRNA LevelsProtein LevelsGene Expression TechniquesFluorescent Substrate BOC-LM-CMACCalpain Activity MeasurementMurine 32Dkit Leukemia CellsSplenocyte Population

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