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High-Resolution Analysis of Mitochondrial Function in Human PBMCs

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Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics

JoVE Journal
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JoVE Journal Biyoloji

Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics

Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics

High-Resolution Analysis of Mitochondrial Function in Human PBMCs

DOI:

10.3791/201849-v

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05:58 min

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May 24, 2024

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Ana P. Valencia, Gavin Pharaoh, Arthur F. Brandao, David J. Marcinek³

¹Division of Metabolism, Endocrinology and Nutrition, Department of Medicine,Washington Üniversitesi, ²Department of Radiology,Washington Üniversitesi, ³Department of Laboratory Medicine and Pathology,Washington Üniversitesi

Etiketler

Magnetic Separation CD14+ Cells CD3+ Cells Fluorespirometry Mitochondrial Dynamics Peripheral Blood Mononuclear Cells (PBMCs)CD14 Microbeads CD3 Microbeads Monocytes T-cells TMRM O2K Respirometer Oxygen Flux Fluorometry

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Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics

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