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Journal
/
Biologie
/
High-Resolution Analysis of Mitochondrial Function in Human PBMCs
/
Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics
Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics
High-Resolution Analysis of Mitochondrial Function in Human PBMCs
DOI:
10.3791/201849-v
•
05:58 min
•
May 24, 2024
•
Ana P. Valencia
,
Gavin Pharaoh
,
Arthur F. Brandao
,
David J. Marcinek
3
1
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine
,
University of Washington
,
2
Department of Radiology
,
University of Washington
,
3
Department of Laboratory Medicine and Pathology
,
University of Washington
Tags
Magnetic Separation
CD14+ Cells
CD3+ Cells
Fluorespirometry
Mitochondrial Dynamics
Peripheral Blood Mononuclear Cells (PBMCs)
CD14 Microbeads
CD3 Microbeads
Monocytes
T-cells
TMRM
O2K Respirometer
Oxygen Flux
Fluorometry
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Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry
Magnetic Separation of CD14+ and CD3+ Cells and Fluorespirometry to Study Mitochondrial Dynamics
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