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High-Quality Nuclei from iPSC-Derived Hematopoietic Cells for Multiomics Studies
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Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells

Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells

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01:20 min

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March 15, 2024

DOI:

10.3791/201642-v

DOI:
10.3791/201642-v

•

01:20 min
March 15, 2024

•

4 Views
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1Division of Neonatology,Children’s Hospital of Philadelphia, 2Pediatri Anabilim Dalı,University of Pennsylvania Perelman School of Medicine

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IPSC-derived Blood CellsNuclei IsolationLive Cell SuspensionDead Cell RemovalBiotin SelectionDextran BeadsCentrifugationDPBSFBSCalcium ChlorideBovine Serum Albumin

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Qiu, R., Petit, C., Thom, C. S. Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells. J. Vis. Exp. (Pending Publication), e201642, doi:10.3791/201642 (2024).

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