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High-Quality Nuclei from iPSC-Derived Hematopoietic Cells for Multiomics Studies
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Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells

Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells

High-Quality Nuclei from iPSC-Derived Hematopoietic Cells for Multiomics Studies

DOI:
10.3791/201642-v

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01:20 min

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March 15, 2024

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, ,
1Division of Neonatology,Children’s Hospital of Philadelphia, 2Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

Tags

IPSC-derived Blood CellsNuclei IsolationLive Cell SuspensionDead Cell RemovalBiotin SelectionDextran BeadsCentrifugationDPBSFBSCalcium ChlorideBovine Serum Albumin

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