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Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

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Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

JoVE Journal
Biyokimya

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JoVE Journal Biyokimya

Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

DOI:

10.3791/200736-v

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02:35 min

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August 25, 2023

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Sarah Wazir, Danny Farhat, Mahalashmi Srinivasan², Jyh-Yeuan Lee

¹Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, ²Department of Biochemistry, Faculty of Medicine and Dentistry,'University of Alberta'

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Reductive Methylation ABCG5/G8 Protein Purification Pre-crystallization Treatment Nickel-NTA Column PD-10 Desalting Cholesterol Addition Ultracentrifugation Protein Concentration Analytical Gel Filtration

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