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Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles
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Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE

Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

DOI:
10.3791/200736-v

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02:35 min

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August 25, 2023

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, , ,
1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, 2Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

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Keywords: Reductive MethylationABCG5/G8 ProteinPurificationPre-crystallization TreatmentNickel-NTA ColumnPD-10 DesaltingCholesterol AdditionUltracentrifugationProtein ConcentrationAnalytical Gel Filtration

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