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In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
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In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
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09:53 min
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July 27, 2010
DOI:
1Interdisciplinary Immunology Program,University of Iowa, and the VA Medical Center, 2Department of Biochemistry,University of Iowa, and the VA Medical Center, 3İç Hastalıkları Anabilim Dalı,University of Iowa, 4Department of Molecular Microbiology,Washington Üniversitesi Tıp Fakültesi, 5Division of Dermatology,Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program,Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology,University of Iowa
Özet
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An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.
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Cite this Article
Thalhofer, C. J., Graff, J. W., Love-Homan, L., Hickerson, S. M., Craft, N., Beverley, S. M., Wilson, M. E. In vivo Imaging of Transgenic Leishmania Parasites in a Live Host. J. Vis. Exp. (41), e1980, doi:10.3791/1980 (2010).
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