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Immunology and Infection

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JoVE Journal Immunology and Infection
In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
 

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Article DOI: 10.3791/1980-v • 09:53 min • July 27th, 2010
July 27th, 2010 •
1, 2, 3, 4, 5, 4, 6,7
1Interdisciplinary Immunology Program, University of Iowa, and the VA Medical Center, 2Department of Biochemistry, University of Iowa, and the VA Medical Center, 3Department of Internal Medicine, University of Iowa, 4Department of Molecular Microbiology, Washington University School of Medicine, 5Division of Dermatology, Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program, Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa

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An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.

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In Vivo Imaging Transgenic Leishmania Parasites Live Host Leishmania Species Visceral Leishmaniasis Cutaneous Leishmaniasis Mouse Models Parasite Burdens Microscopy Limiting Dilution Assay QPCR Amplification In Vivo Imaging System (IVIS) Bioluminescent Signal Luciferase Expression Intradermal Injection Intravenous Injection Longitudinal Studies Inter-animal Variation Parasite Inocula Multiplication Of Parasites Mouse Tissues High Sensitivity Cutaneous Locations
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