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Microinjection of Medaka Embryos for use as a Model Genetic Organism
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December 22, 2010
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Medaka and zebrafish are complementary for genetic dissection of vertebrate genome functions. This protocol highlights the key points for successful microinjection into medaka embryos, an important technique for embryological and genetic analysis using medaka and zebrafish in a laboratory.
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MicroinjectionMedaka EmbryosModel Genetic OrganismVertebrate Model OrganismsGenetic AnalysisEmbryological AnalysisPhenotype-driven Mutant ScreensZebrafishMouseFunctional OverlapNovel PhenotypesGenetic DissectionVertebrate Genome FunctionsCell-tracersLabeling CellsMRNAAnti-sense OligonucleotidesGene ExpressionTransgenic Lines
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Porazinski, S. R., Wang, H., Furutani-Seiki, M. Microinjection of Medaka Embryos for use as a Model Genetic Organism. J. Vis. Exp. (46), e1937, doi:10.3791/1937 (2010).
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