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Microinjection of Medaka Embryos for use as a Model Genetic Organism
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生物学
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JoVE Journal 生物学
Microinjection of Medaka Embryos for use as a Model Genetic Organism

Microinjection of Medaka Embryos for use as a Model Genetic Organism

DOI:
10.3791/1937-v

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07:25 min

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December 22, 2010

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, ,
1Centre for Regenerative Medicine, Department of Biology and Biochemistry,University of Bath

章

  • 00:05標題
  • 00:55Introduction
  • 01:28Mating and Egg Collection
  • 02:58Making Agarose Plates for Holding Embryos
  • 03:58Microinjection of Medaka Embryos
  • 06:14Representative Images of Microinjected Embryos
  • 06:59Conclusion

概要

自動翻訳

自動翻訳

Medaka and zebrafish are complementary for genetic dissection of vertebrate genome functions. This protocol highlights the key points for successful microinjection into medaka embryos, an important technique for embryological and genetic analysis using medaka and zebrafish in a laboratory.

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MicroinjectionMedaka EmbryosModel Genetic OrganismVertebrate Model OrganismsGenetic AnalysisEmbryological AnalysisPhenotype-driven Mutant ScreensZebrafishMouseFunctional OverlapNovel PhenotypesGenetic DissectionVertebrate Genome FunctionsCell-tracersLabeling CellsMRNAAnti-sense OligonucleotidesGene ExpressionTransgenic Lines

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