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Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
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12:23 min
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May 25, 2010
DOI:
1Molecular Biology of Neural Development,Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering,McGill University, 3Program in Neuroengineering,McGill University, 4Montreal Neurological Institute, 5Anatomi ve Hücre Biyolojisi Anabilim Dalı,McGill University, 6Biyoloji Bölümü,McGill University, 7Tıp Fakültesi,Universite de Montreal – University of Montreal
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This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.
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Langlois, S. D., Morin, S., Yam, P. T., Charron, F. Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord. J. Vis. Exp. (39), e1773, doi:10.3791/1773 (2010).
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