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Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
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Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

16,515 Views

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12:23 min

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May 25, 2010

DOI:

10.3791/1773-v

DOI:
10.3791/1773-v

•

12:23 min
May 25, 2010

•

8 Views
, , ,
1Molecular Biology of Neural Development,Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering,McGill University, 3Program in Neuroengineering,McGill University, 4Montreal Neurological Institute, 5解剖・細胞生物学科,McGill University, 6生物学科,McGill University, 7医学部,Universite de Montreal – University of Montreal

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This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.

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DissectionCultureCommissural NeuronsEmbryonic Spinal CordAxon GuidanceDorsal Spinal CordStereotyped TrajectoriesGuidance CuesTurning AssaysImmunochemistry生化学E13 RatDissociationCell SuspensionPoly-L-lysine-coated Glass CoverslipsIn Vitro StudiesCellular MechanismsMolecular Mechanisms

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Langlois, S. D., Morin, S., Yam, P. T., Charron, F. Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord. J. Vis. Exp. (39), e1773, doi:10.3791/1773 (2010).

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