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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

DOI:
10.3791/1514-v

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09:00 min

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August 04, 2009

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1Sigma Aldrich

Bölümler

  • 00:05Başlık
  • 00:59Introduction
  • 01:21The Protease Assay
  • 06:41Fluorescence Measurement
  • 07:43Fluorescence Measurement of Protease Activity Representative Results
  • 08:29Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.

Etiketler

ProteaseFluorescent Detection KitProtease ActivityAssayCasein SubstrateFluorescein Isothiocyanate (FITC)CleavageAcidificationTrichloroacetic Acid (TCA)CentrifugationPelletAcid Soluble FragmentsSupernatantNeutralizationFluorescence MeasurementTrypsin Protease ControlConcentrationIncubation TimeMicrocentrifuge TubesCuvettesMultiwell Plates

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