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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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August 04, 2009
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ProteaseFluorescent Detection KitProtease ActivityAssayCasein SubstrateFluorescein Isothiocyanate (FITC)CleavageAcidificationTrichloroacetic Acid (TCA)CentrifugationPelletAcid Soluble FragmentsSupernatantNeutralizationFluorescence MeasurementTrypsin Protease ControlConcentrationIncubation TimeMicrocentrifuge TubesCuvettesMultiwell Plates
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Cupp-Enyard, C. Use of the Protease Fluorescent Detection Kit to Determine Protease Activity. J. Vis. Exp. (30), e1514, doi:10.3791/1514 (2009).
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