Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs

Published: February 29, 2024

Abstract

Source: Babini, G., et al. A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC. J. Vis. Exp. (2018)

This video demonstrates the measurement of trans-epithelial electrical resistance in irradiated Caco-2 cells, both in the presence and absence of PBMC cells. This method enables the study of the synergistic effects of radiation and immune cells on the epithelial cell barrier.

Protocol

1. Irradiation Set-up Note: Irradiation of Caco-2 cells was performed at the radiotherapy department of Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) S. Maugeri (Pavia, Italy) with a linear accelerator routinely used to treat different types of cancers. Set photon X-ray energy to 6 MV peak. The linear accelerator operates in a pulsing regime (time between two consecutive pulses approximately of 4 ms; duration of a single pulse about 5…

Açıklamalar

The authors have nothing to disclose.

Materials

ThinCert 6 Well Cell Culture Inserts for Multiwell Plates Greiner Bio-one 657610 Equipment
Cell Culture Multiwell Plate, 6 Well Greiner Bio-one 657160 Plastic
Cell Culture Multiwell Plate, 24 Well Greiner Bio-one 662160 Plastic
RPMI 1640 without L-Glutamine Lonza 12-167F Reagents
Millicell-ERS voltmeter/ohmeter Millipore MERS 00001 Equipment

Etiketler

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Bu Makaleden Alıntı Yapın
Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs. J. Vis. Exp. (Pending Publication), e21979, doi: (2024).

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