Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs
Published: February 29, 2024
Abstract
Source: Babini, G., et al. A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC. J. Vis. Exp. (2018)
This video demonstrates the measurement of trans-epithelial electrical resistance in irradiated Caco-2 cells, both in the presence and absence of PBMC cells. This method enables the study of the synergistic effects of radiation and immune cells on the epithelial cell barrier.
Protocol
1. Irradiation Set-up Note: Irradiation of Caco-2 cells was performed at the radiotherapy department of Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) S. Maugeri (Pavia, Italy) with a linear accelerator routinely used to treat different types of cancers. Set photon X-ray energy to 6 MV peak. The linear accelerator operates in a pulsing regime (time between two consecutive pulses approximately of 4 ms; duration of a single pulse about 5…
Açıklamalar
The authors have nothing to disclose.
Materials
| ThinCert 6 Well Cell Culture Inserts for Multiwell Plates | Greiner Bio-one | 657610 | Equipment |
| Cell Culture Multiwell Plate, 6 Well | Greiner Bio-one | 657160 | Plastic |
| Cell Culture Multiwell Plate, 24 Well | Greiner Bio-one | 662160 | Plastic |
| RPMI 1640 without L-Glutamine | Lonza | 12-167F | Reagents |
| Millicell-ERS voltmeter/ohmeter | Millipore | MERS 00001 | Equipment |
Etiketler
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Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs. J. Vis. Exp. (Pending Publication), e21979, doi: (2024).