Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis

Published: April 30, 2023

Abstract

Source: Nicholson, T. M. et al. Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia. J. Vis. Exp. (2013)

In this video, we demonstrate the implantation of human-derived prostate cancer tissue grafts into the renal capsule of an immunocompromised recipient mouse. This technique can be used to assess tumor progression and develop novel therapeutic strategies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Surgical Procedure: Renal Capsule Xenografting Prior to induction of anesthesia, observe the mouse to ensure health and wellbeing. Place the mouse into the chamber for induction of isoflurane anesthesia at 3-5%. When the animal has stopped moving and the respiratory rate has decreased, transfer to the nose con…

Açıklamalar

The authors have nothing to disclose.

Materials

Sterile surgical gown Midwest Vet Supply 350.79866.2
Surgical mask Midwest Vet Supply 350.50111.2
Sterile Gloves  Kimberly Clark  55092
Reflex Wound Clipper  F.S.T.  12031-09 Correspond w/ wound clip size
Vicryl Suture  Midwest Vet Supply 295-92100.2  4-0, FS-2, Absorbable
Straight Sharp/Blunt Scissors  Fine Scientific Tools (F.S.T.) 14054-13
Graefe forceps (Serrated, Toothed, Curved) F.S.T. 11055-10
Isoflurane Vaporizer  Supera Anesthesia Innov VAP3000
Induction Chamber  Supera Anesthesia Innov RES644
Glass Pasteur pippets  Fisher Scientific S67050 5" length
Graefe forceps (Serrated, Curved)  F.S.T. 11052-10
Graefe forceps (Serrated, Straight) F.S.T. 11050-10
Betadine  Webster Veterinary 07-836-3379
Sterile saline Midwest Vet Supply 193.74504.3  NaCl 0.9%, Injectable
Cotton Tip Applicator  Midwest Vet Supply 001.06220.2
Wound Clips  Braintree Scientific  ACS CS May use 7mm or 9mm clips
Isoflurane  Midwest Vet Supply 193.33161.3
Dissecting microscope  Leica  L2
Small Mice Nose Cone  Supera Anesthesia Innov  ACC526
Oxygen Flowmeter  Supera Anesthesia Innov  OXY660
Would Clip Remover  F.S.T.  12033-00  Universal

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Bu Makaleden Alıntı Yapın
Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis. J. Vis. Exp. (Pending Publication), e20401, doi: (2023).

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