Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis
Published: April 30, 2023
Abstract
Source: Nicholson, T. M. et al. Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia. J. Vis. Exp. (2013)
In this video, we demonstrate the implantation of human-derived prostate cancer tissue grafts into the renal capsule of an immunocompromised recipient mouse. This technique can be used to assess tumor progression and develop novel therapeutic strategies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Surgical Procedure: Renal Capsule Xenografting Prior to induction of anesthesia, observe the mouse to ensure health and wellbeing. Place the mouse into the chamber for induction of isoflurane anesthesia at 3-5%. When the animal has stopped moving and the respiratory rate has decreased, transfer to the nose con…
Açıklamalar
The authors have nothing to disclose.
Materials
| Sterile surgical gown | Midwest Vet Supply | 350.79866.2 | |
| Surgical mask | Midwest Vet Supply | 350.50111.2 | |
| Sterile Gloves | Kimberly Clark | 55092 | |
| Reflex Wound Clipper | F.S.T. | 12031-09 | Correspond w/ wound clip size |
| Vicryl Suture | Midwest Vet Supply | 295-92100.2 | 4-0, FS-2, Absorbable |
| Straight Sharp/Blunt Scissors | Fine Scientific Tools (F.S.T.) | 14054-13 | |
| Graefe forceps (Serrated, Toothed, Curved) | F.S.T. | 11055-10 | |
| Isoflurane Vaporizer | Supera Anesthesia Innov | VAP3000 | |
| Induction Chamber | Supera Anesthesia Innov | RES644 | |
| Glass Pasteur pippets | Fisher Scientific | S67050 | 5" length |
| Graefe forceps (Serrated, Curved) | F.S.T. | 11052-10 | |
| Graefe forceps (Serrated, Straight) | F.S.T. | 11050-10 | |
| Betadine | Webster Veterinary | 07-836-3379 | |
| Sterile saline | Midwest Vet Supply | 193.74504.3 | NaCl 0.9%, Injectable |
| Cotton Tip Applicator | Midwest Vet Supply | 001.06220.2 | |
| Wound Clips | Braintree Scientific | ACS CS | May use 7mm or 9mm clips |
| Isoflurane | Midwest Vet Supply | 193.33161.3 | |
| Dissecting microscope | Leica | L2 | |
| Small Mice Nose Cone | Supera Anesthesia Innov | ACC526 | |
| Oxygen Flowmeter | Supera Anesthesia Innov | OXY660 | |
| Would Clip Remover | F.S.T. | 12033-00 | Universal |
Bu Makaleden Alıntı Yapın
Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis. J. Vis. Exp. (Pending Publication), e20401, doi: (2023).