Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology

Published: April 30, 2023

Abstract

Source: Nagaraj A.S., et al., Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing. J. Vis. Exp. (2018)

The following video describes how to preserve morphology of a tissue with formalin fixation and paraffin embedding.

Protocol

1. Fixation and Processing of the Tissue Slices Carefully lift the uncultured 0 h reference or cultured slice onto a filter paper soaked in PBS. To do this, add 2-3 mL of PBS on top of a filter paper placed in a 10 cm plate. Place the slice on top of the filter paper and lift out the filter paper using a pair of forceps. Transfer the filter paper into a histocassette, and add a drop of diluted hematoxylin (1:1 in deionized water) on top of the tissue slice to visibly mark the position o…

Representative Results

Figure 1: Schematic representation of the workflow for establishment and analysis of murine NSCLC tumor-derived slice explants. (A) Schematic describing the collection and preparation of tumor-bearing lungs for slicing. Lung lobes are harvested from a mouse and tumor tissue is dissected away from normal tissue. The black arrowhead and asterisk indicate approximately 4 mm and…

Açıklamalar

The authors have nothing to disclose.

Materials

10 cm tissue culture plate  Sarstedt  83.1802
PBS  Lonza  BE17-517Q
Formaldehyde  Fisher  F/1501/PB08
Trifold histo cassette paper  Cancer Diagnostics  DX26280
Histo cassettes  VWR  720-2199
Microtome  Thermo Fischer Scientific  HM355S
Leica VT1200 S vibrating blade microtome Leica Biosystems 14048142066
Hematoxylin for H&E staining Merck 1.09249.0500
Hematoxylin for counter staining Dako S3309
Eosin Sigma E4382
Hank’s Balanced salt solution (HBSS) Sigma H6648

Play Video

Bu Makaleden Alıntı Yapın
Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology. J. Vis. Exp. (Pending Publication), e20255, doi: (2023).

View Video