Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology
Published: April 30, 2023
Abstract
Source: Nagaraj A.S., et al., Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing. J. Vis. Exp. (2018)
The following video describes how to preserve morphology of a tissue with formalin fixation and paraffin embedding.
Protocol
1. Fixation and Processing of the Tissue Slices Carefully lift the uncultured 0 h reference or cultured slice onto a filter paper soaked in PBS. To do this, add 2-3 mL of PBS on top of a filter paper placed in a 10 cm plate. Place the slice on top of the filter paper and lift out the filter paper using a pair of forceps. Transfer the filter paper into a histocassette, and add a drop of diluted hematoxylin (1:1 in deionized water) on top of the tissue slice to visibly mark the position o…
Representative Results
Figure 1: Schematic representation of the workflow for establishment and analysis of murine NSCLC tumor-derived slice explants. (A) Schematic describing the collection and preparation of tumor-bearing lungs for slicing. Lung lobes are harvested from a mouse and tumor tissue is dissected away from normal tissue. The black arrowhead and asterisk indicate approximately 4 mm and…
Açıklamalar
The authors have nothing to disclose.
Materials
| 10 cm tissue culture plate | Sarstedt | 83.1802 | |
| PBS | Lonza | BE17-517Q | |
| Formaldehyde | Fisher | F/1501/PB08 | |
| Trifold histo cassette paper | Cancer Diagnostics | DX26280 | |
| Histo cassettes | VWR | 720-2199 | |
| Microtome | Thermo Fischer Scientific | HM355S | |
| Leica VT1200 S vibrating blade microtome | Leica Biosystems | 14048142066 | |
| Hematoxylin for H&E staining | Merck | 1.09249.0500 | |
| Hematoxylin for counter staining | Dako | S3309 | |
| Eosin | Sigma | E4382 | |
| Hank’s Balanced salt solution (HBSS) | Sigma | H6648 |
Bu Makaleden Alıntı Yapın
Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology. J. Vis. Exp. (Pending Publication), e20255, doi: (2023).