JOVE JOURNAL

Biyokimya

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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Danlin Yang¹, Ajit Singh², Helen Wu¹, Rachel Kroe-Barrett¹

¹Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., ²The Fu Foundation School of Engineering and Applied Science,Columbia University

Journal (Biochemistry)

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Kathrin Rösch¹, Marcel Kwiatkowski², Hartmut Schlüter², Eva Herker¹

¹Heinrich Pette Institute,Leibniz Institute for Experimental Virology, ²Core Facility Mass Spectrometric Proteomics,University Medical Center Hamburg-Eppendorf

Journal (Biochemistry)

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Cristina Lento¹, Shaolong Zhu¹, Kerene A. Brown¹, Ruth Knox¹, Peter Liuni¹, Derek J. Wilson^1,2,3

¹Kimya Bölümü,York University, ²The Centre for Research in Mass Spectrometry,York University, ³The Centre for Research on Biomolecular Interactions,York University

Journal (Biochemistry)

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

Xavier Gallart-Palau*¹, Aida Serra*¹, Siu Kwan Sze¹

¹Division of Structural Biology and Biochemistry, School of Biological Sciences,Nanyang Technological University

Journal (Biochemistry)

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

Cory A. Bontrager¹, Tanner J. Geibel¹, George A. Lengyel¹

¹Kimya Bölümü,Slippery Rock University

Journal (Biochemistry)

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Mercedes Pardo¹, Daniel Bode¹, Lu Yu¹, Jyoti S. Choudhary¹

¹Proteomic Mass Spectrometry,Wellcome Trust Sanger Institute

Journal (Biochemistry)

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

David J. Samuels¹, Zhe Wang², Kyu Y. Rhee^2,3, Shaun R. Brinsmade¹

¹Biyoloji Bölümü,Georgetown University, ²Division of Infectious Diseases,Weill Cornell Medical College, ³Tıp Fakültesi,Weill Cornell Medical College

Journal (Biochemistry)

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Andrew Baxter¹, Emmanuel Minet¹

¹Research and Development,British American Tobacco

Journal (Biochemistry)

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Christopher J. A. Warner¹, Subrata Dutta¹, Alejandro R. Foley¹, Jevgenij A. Raskatov¹

¹Kimya Bölümü,University of California, Santa Cruz

Journal (Biochemistry)

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Stamatios Liokatis¹

¹Department of Structural Biology,Leibniz Institute of Molecular Pharmacology (FMP-Berlin)

Journal (Biochemistry)

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Hyelee Lee¹, Shih-Yuan Liu¹

¹Kimya Bölümü,Boston College

Journal (Biochemistry)

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Eric D. Routh*¹, Steven D. Creacy*², Peter E. Beerbower³, Steven A. Akman⁴, James P. Vaughn¹, Philip J. Smaldino³

¹Department of Cancer Biology,Wake Forest School of Medicine, ²YX Genomics, ³Biyoloji Bölümü,Ball State University, ⁴Department of Hematology and Oncology,Roper St. Francis Hospital

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