JOVE JOURNAL

Biyokimya

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Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Yu Han^1,3, Cody T. Thomas^1,3, Sara A. Wennersten^1,3, Edward Lau^1,3, Maggie P. Y. Lam^1,2,3

¹Department of Medicine-Cardiology,University of Colorado Anschutz Medical Campus, ²Department of Biochemistry & Molecular Genetics,University of Colorado Anschutz Medical Campus, ³Consortium for Fibrosis Research & Translation, School of Medicine,University of Colorado Anschutz Medical Campus

Journal (Biochemistry)

Quaternary Structure Modeling Through Chemical Cross-Linking Mass Spectrometry: Extending TX-MS Jupyter Reports

Hamed Khakzad^1,2, Swen Vermeul³, Lars Malmström^4,5,6

¹Equipe Signalisation Calcique et Infections Microbiennes,Ecole Normale Supérieure Paris-Saclay, ²Institut National de la Santé et de la Recherche Médicale, ³Scientific IT Services,ETH Zurich, ⁴Institute for Computational Science,University of Zurich, ⁵S3IT,University of Zurich, ⁶Division of Infection Medicine, Department of Clinical Sciences Lund, Faculty of Medicine,Lund University

Journal (Biochemistry)

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

David Vanderwall¹, Poudel Suresh^1,2, Yingxue Fu², Ji-Hoon Cho², Timothy I. Shaw^2,3, Ashutosh Mishra², Anthony A. High², Junmin Peng^1,2, Yuxin Li^1,2

¹Departments of Structural Biology and Developmental Neurobiology,St. Jude Children’s Research Hospital, ²Center for Proteomics and Metabolomics,St. Jude Children’s Research Hospital, ³Department of Computational Biology,St. Jude Children’s Research Hospital

Journal (Biochemistry)

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Isabelle Sirois*¹, Maxim Isabelle*¹, Jérôme D. Duquette¹, Frederic Saab¹, Etienne Caron^1,2

¹CHU Sainte-Justine Research Center, ²Department of Pathology and Cellular Biology, Faculty of Medicine,Université de Montréal

Journal (Biochemistry)

Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees

Yingying Song¹, Ruiping Li^1,2, Lili Li¹, Fang Ouyang³, Xingyuan Men¹

¹Institute of Plant Protection,Shandong Academy of Agricultural Sciences, ²College of Plant Protection,Shandong Agricultural University, ³State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology,Chinese Academy of Sciences

Journal (Biochemistry)

Chemical Modification of the Tryptophan Residue in a Recombinant Ca²⁺-ATPase N-domain for Studying Tryptophan-ANS FRET

José G. Sampedro¹, Yolanda Cataño¹

¹Instituto de Física,Universidad Autónoma de San Luis Potosí

Journal (Biochemistry)

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Courtney N. Johnson¹, David S. Libich¹

¹Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute,The University of Texas Health Science Center at San Antonio

Journal (Biochemistry)

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Yonatan R. Lewis-Israeli^1,2, Brett D. Volmert^1,2, Mitchell A. Gabalski^1,2, Amanda R. Huang^1,2, Aitor Aguirre^1,2

¹Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology,Michigan State University, ²Department of Biomedical Engineering, College of Engineering,Michigan State University

Journal (Biochemistry)

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Jason Wong¹, William Martelly¹, Shalini Sharma¹

¹Department of Basic Medical Sciences,College of Medicine-Phoenix, University of Arizona

Journal (Biochemistry)

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Paul-Lennard Mendez^1,2, Leon Obendorf¹, Petra Knaus¹

¹Institute for Chemistry and Biochemistry,Freie Universität Berlin, ²International Max-Planck Research School for Biology and Computation

Journal (Biochemistry)

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Bryan S. Sibert*^1,2,3, Joseph Y. Kim*^1,4, Jie E. Yang^1,2,3, Elizabeth R. Wright^1,2,3,5

¹Department of Biochemistry,University of Wisconsin, Madison, ²Cryo-Electron Microscopy Research Center, Department of Biochemistry,University of Wisconsin, Madison, ³Midwest Center for Cryo-Electron Tomography, Department of Biochemistry,University of Wisconsin, Madison, ⁴Kimya Bölümü,University of Wisconsin, Madison, ⁵Morgridge Institute for Research

Journal (Biochemistry)

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells

Jaime Padros¹, Geneviève Chatel¹, Mireille Caron¹

¹BioAuxilium Research

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