An In Situ Immunostaining Protocol for Parkinson's Disease Study

June 23, 2023

帕金森病模型中线粒体形态的组织学检查

通过免疫组化对α-突触核蛋白预成型原纤维（PFF）注射小鼠石蜡包埋脑切片进行线粒体形态分析

Dalila Ciceri*¹, Lierni Gregorio-Zabala*¹, Xabier Llama-Pino, Begüm Kurt, Jon Olano-Bringas, Patricia Villegas-Zafra, Nora Bengoa-Vergniory^2,3,4

¹Achucarro Basque Center for Neuroscience, ²Nörobilim Bölümü,University of the Basque Country (UPV/EHU), ³Ikerbasque – Basque Foundation for Science, ⁴Oxford Parkinson’s Disease Centre and Department of Physiology, Anatomy and Genetics,University of Oxford

Özet

本研究提出了一种基于免疫染色和图像分析的小鼠脑组织原位线粒体形态分析方法。它还描述了这如何允许人们检测帕金森病模型中蛋白质聚集引起的线粒体形态的变化。

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