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Neuroscienze
An In Situ Immunostaining Protocol for Parkinson's Disease Study

An In Situ Immunostaining Protocol for Parkinson's Disease Study

June 23, 2023

Author Spotlight: Establishing a New Fluorescence-Based Protocol for In Vivo Mitochondrial Morphology Analysis in Parkinson's Disease

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Mitochondrial Morphology Analysis by Immunohistochemistry on Paraffin-Embedded Brain Slices from α-Synuclein Pre-Formed Fibrils (PFF)-Injected Mice

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1Achucarro Basque Center for Neuroscience, 2Department of Neuroscience,University of the Basque Country (UPV/EHU), 3Ikerbasque – Basque Foundation for Science, 4Oxford Parkinson’s Disease Centre and Department of Physiology, Anatomy and Genetics,University of Oxford

Summary

This study presents a method to analyze the morphology of mitochondria based on immunostaining and image analysis in mouse brain tissue in situ. It also describes how this allows one to detect changes in mitochondrial morphology induced by protein aggregation in Parkinson’s disease models.

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