Adult Zebrafish Electrophysiology: A Method to Measure the Extracellular Electrical Activity of Neurons In Vivo
Published: April 30, 2023
Abstract
Source: Johnston, L., et al. Electrophysiological Recording in the Brain of Intact Adult Zebrafish. J. Vis. Exp. (2013)
The protocol describes the procedure to perform electrophysiology in adult zebrafish. This method can be used for measuring neural activity in vivo.
Protocol
1. Electrophysiology Shut off the intubation stop-cock and quickly disconnect the Luer lock connecting the intubation base to the tricaine drip. Move the intact intubation setup to the electrophysiology microscope and connect to the perfusion system. Turn on the habitat water and perfuse at a rate of 1 ml/min for ~1 hr, in order to wash out the tricaine. Using a micromanipulator under visual control, position the secondary electrode so that the electrode tip…
Materials
| Habitat water | pH 7.0-7.4, conductivity of 400-450 μS; maintained by Instant Ocean and Sodium Bicarbonate |
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| 0.4% Tris-Buffered Tricaine | Sigma-Aldrich | E10521 | pH 7.2-7.4; stored at -20 oC |
| 60 ml Luer lock syringe tubes | Becton, Dickinson and Company | 309653 | |
| 3-way Stopcocks with Luer connections | |||
| 0.010 in Silver wire | A-M Systems, Inc. | ||
| Q-series electrode holder | |||
| Fisherbrand 100 mm x 15 mm Petri dish | Fisher Scientific | NC9299146 | |
| 34 G Beveled needle | World Precision Instruments, Inc. | NF34BV | |
| 37 cm x 42 cm Kimwip | Kimberly-Clark Professional | TW31KEM | |
| 1/8 in diameter tube | |||
| 1 mm diameter tube | |||
| Microscope (Leica MZ APO) | Another microscope can be used |
Cite This Article
Adult Zebrafish Electrophysiology: A Method to Measure the Extracellular Electrical Activity of Neurons In Vivo. J. Vis. Exp. (Pending Publication), e20192, doi: (2023).