JOVE JOURNAL

Immunology and Infection

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Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Qin Xiao*¹, Yuheng Liu*¹, Dandan Zhang, Chao Li, Qihua Yang, Dongshui Lu, Weijun Zhang, Joseph Rosenecker, Quanming Zou, Yang Li, Shan Guan

¹National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, ²Department of Pediatrics,Ludwig-Maximilians University of Munich, ³Department of Pharmacy, Southwest Hospital,Third Military Medical University

Journal (Immunology and Infection)

In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

Luna de Lacerda*¹, Guilherme Castro*^1,2, Cristopher Gomes, Caroline Junqueira

¹Instituto René Rachou,Fundação Oswaldo Cruz, ²Departamento de Bioquímica e Imunologia,Universidade Federal de Minas Gerais

Journal (Immunology and Infection)

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Melissa Berni, Stefano Pongolini, Martina Tambassi

¹Risk Analysis and Genomic Epidemiology Unit,Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna (IZSLER)

Journal (Immunology and Infection)

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Jeeshan Singh², Xiaomei Shan², Aparna Mahajan², Martin Herrmann², Christine Schauer², Jasmin Knopf², Luis E. Muñoz²

¹Department of Internal Medicine 3-Rheumatology and Immunology,Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, ²Deutsches Zentrum für Immuntherapie,Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen

Journal (Immunology and Infection)

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

Avik Chattopadhyay, Joel P. Joseph, Sai Shyam, Dipankar Nandi

¹Department of Biochemistry,Indian Institute of Science, ²Center for BioSystems Science and Engineering,Indian Institute of Science, ³Undergraduate Program,Indian Institute of Science

Journal (Immunology and Infection)

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Fei Wang*¹, Cihan Yang*^1,2, Shunlong Wang², Qun Wu², Christabel Ochieng², Zhiming Yuan², Han Xia²

¹Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology,Chinese Academy of Sciences, ²University of Chinese Academy of Sciences

Journal (Immunology and Infection)

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Heather Liebe, Camille Schlegel, Xue Cai, Alena Golubkova, Tyler Leiva, William L. Berry, Catherine J. Hunter

¹Division of Pediatric Surgery,Oklahoma Children's Hospital, ²Oklahoma University Health Sciences Center, ³Department of Surgery,University of Oklahoma Health Sciences Center

Journal (Immunology and Infection)

RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

Dalaena E. Rivera, Vladimir Lažetić, Emily R. Troemel, Robert J. Luallen

¹Department of Biology,San Diego State University, ²School of Biological Sciences,University of California, San Diego

Journal (Immunology and Infection)

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

William K. Boyle, Hannah N. Sorensen, Travis J. Bourret

¹School of Medicine,Creighton University

Journal (Immunology and Infection)

A Modified Surgical Technique for Kidney Transplantation in Mice

Decheng Yin*^1,2, Jian Fu*^1,3, Rongjun Chen, Nelli Shushakova, Ida Allabauer, Xin-Yi Wei, Mario Schiffer, Diana Dudziak, Song Rong*⁵, André Hoerning*¹

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery,Chengdu Women’s and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue,The first affiliated Hospital of Hainan Medical University, ⁴Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases,Hannover Medical School, ⁵Department of Nephrology,Hannover Medical School, ⁶Department of Nephrology, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg, ⁷Department of Dermatology, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg

Journal (Immunology and Infection)

Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection

Matloob Husain

¹Department of Microbiology and Immunology,University of Otago

Journal (Immunology and Infection)

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Xiaofan Jia*¹, Ling He*^1,2, Dongmei Miao, Caiguo Zhang, Marian Rewers, Liping Yu

¹Barbara Davis Center for Diabetes,University of Colorado School of Medicine, ²Department of Endocrinology, Guangzhou First People’s Hospital, School of Medicine,South China University of Technology

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