JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Tiffany Snow¹, Jon Roussey¹, Casey Wegner¹, Brandon McNaughton²

¹Research and Development,Akadeum Life Sciences, ²Akadeum Life Sciences

Journal (Immunology and Infection)

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Loni Perrenoud¹, James Conley¹, Leslie J. Berg¹

¹Department of Immunology and Microbiology,University of Colorado-Anschutz School of Medicine

Journal (Immunology and Infection)

Establishment of Deep Hypothermic Circulatory Arrest in Rats

Weidong Yan¹, Bingyang Ji¹

¹Department of Cardiopulmonary Bypass, National Center for Cardiovascular Disease and Fuwai Hospital, State Key Laboratory of Cardiovascular Medicine, Chinese Academy of Medical Science,Peking Union Medical College

Journal (Immunology and Infection)

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

B. Thomas Bäckström^1,2

¹The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences,Lund University, ²Department of Autoimmunity,BTB Emulsions

Journal (Immunology and Infection)

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Xing Luo¹, Yanan Tong¹, Xiaoqiang Zeng¹, Yan Ye¹, Yun Yang¹, Zhen Song¹, Zelong Zhang¹, Haibo Li¹, Jining Gao², Xuhu Mao¹, Hao Zeng¹, Quanming Zou¹, Hongwu Sun¹

¹Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy,Third Military Medical University of Chinese PLA, ²Institute of Combined Injury of PLA, College of Military Preventive Medicine,Third Military Medical University of Chinese PLA

Journal (Immunology and Infection)

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Li Yaozhen¹, Wu Kemin¹, Ji Xiaoyu¹, Ouyang Yang¹, Tang Hongying¹, Pan Baihong¹

¹Department of Vascular Surgery, Xiangya Hospital,Central South University

Journal (Immunology and Infection)

Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia

Anne Berthold*¹, Marie-Line Faucillion*², Ingela Nilsson², Maryna Golovchenko³, Vett Lloyd¹, Sven Bergström², Natalie Rudenko³

¹Department of Biology,Mt. Allison University, ²Department of Molecular Biology,Umeå University, ³Biology Centre Czech Academy of Sciences,Institute of Parasitology

Journal (Immunology and Infection)

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Alexia Almaraz-Arreortua¹, Sorely Adelina Sosa-Luis², William de Jesús Ríos-Ríos¹, María de los Ángeles Romero-Tlalolini³, Sergio Roberto Aguilar-Ruiz⁴, Rafael Baltiérrez-Hoyos³, Honorio Torres Aguilar¹

¹Clinical Immunology Research Department of Biochemical Sciences Faculty,Universidad Autónoma “Benito Juárez” de Oaxaca, ²Department of Molecular Biomedicine,Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, ³CONACYT- Medicine and Surgery Faculty,Universidad Autónoma “Benito Juárez” de Oaxaca, ⁴Molecular Immunology Research Department of Medicine and Surgery Faculty,Universidad Autónoma “Benito Juárez” de Oaxaca

Journal (Immunology and Infection)

Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

Chaitanya S. Haram¹, Samrat Moitra¹, Rilee Keane¹, Elana Breslav¹, Kai Zhang¹, Peter A. Keyel¹

¹Department of Biological Sciences,Texas Tech University

Journal (Immunology and Infection)

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Kristina Irsch^1,2,3, Kate Grieve^1,2, Marie Borderie², Maëlle Vilbert^1,2,4, Karsten Plamann^4,5, Djida Ghoubay^1,2, Cristina Georgeon², Vincent Borderie^1,2

¹Vision Institute,Sorbonne University, UM 80 / INSERM, UMR_S 968 / CNRS, UMR_7210, ²Quinze Vingts National Ophthalmology Hospital, ³Laboratory of Ophthalmic Instrument Development, The Wilmer Eye Institute,Johns Hopkins University School of Medicine, ⁴Laboratory for Optics and Biosciences (LOB),École polytechnique, ⁵LOA - ENSTA Paris,École polytechnique

Journal (Immunology and Infection)

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Josephine Nemegeer^1,2, Kelly Lemeire^1,2, Peter Vandenabeele^1,2, Jonathan Maelfait^1,2

¹VIB-UGent Center for Inflammation Research, ²Department of Biomedical Molecular Biology,Ghent University

Journal (Immunology and Infection)

Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Rohan S. Wakade¹, Damian J. Krysan^1,2, Melanie Wellington¹

¹Department of Pediatrics, Carver College of Medicine,University of Iowa, ²Department of Microbiology and Immunology, Carver College of Medicine,University of Iowa

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