JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

Dalaena E. Rivera¹, Vladimir Lažetić², Emily R. Troemel², Robert J. Luallen¹

¹Department of Biology,San Diego State University, ²School of Biological Sciences,University of California, San Diego

Journal (Immunology and Infection)

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

William K. Boyle¹, Hannah N. Sorensen¹, Travis J. Bourret¹

¹School of Medicine,Creighton University

Journal (Immunology and Infection)

A Modified Surgical Technique for Kidney Transplantation in Mice

Decheng Yin*^1,2, Jian Fu*^1,3, Rongjun Chen⁴, Nelli Shushakova⁵, Ida Allabauer¹, Xin-Yi Wei¹, Mario Schiffer⁶, Diana Dudziak⁷, Song Rong*⁵, André Hoerning*¹

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery,Chengdu Women’s and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue,The first affiliated Hospital of Hainan Medical University, ⁴Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases,Hannover Medical School, ⁵Department of Nephrology,Hannover Medical School, ⁶Department of Nephrology, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg, ⁷Department of Dermatology, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg

Journal (Immunology and Infection)

Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection

Matloob Husain¹

¹Department of Microbiology and Immunology,University of Otago

Journal (Immunology and Infection)

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Xiaofan Jia*¹, Ling He*^1,2, Dongmei Miao¹, Caiguo Zhang¹, Marian Rewers¹, Liping Yu¹

¹Barbara Davis Center for Diabetes,University of Colorado School of Medicine, ²Department of Endocrinology, Guangzhou First People’s Hospital, School of Medicine,South China University of Technology

Journal (Immunology and Infection)

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Huiqin Tang*¹, Hui Xie*¹, Zhun Wang¹, Shuanghe Peng², Wenli Ni¹, Linpei Guo³

¹Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, ²Department of Pathology,The Second Hospital of Tianjin Medical University, ³Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Journal (Immunology and Infection)

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Madison I. Moss¹, Mariela Pauli², Joshua M. Moreau¹, Jarish N. Cohen¹, Michael D. Rosenblum¹, Margaret M. Lowe¹

¹Department of Dermatology,UCSF, ²TRex Bio

Journal (Immunology and Infection)

Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

Wenjun Li¹, Hailey M. Shepherd¹, Alexander S. Krupnick², Andrew E. Gelman^1,3, Kory J. Lavine⁴, Daniel Kreisel^1,3

¹Department of Surgery,Washington University School of Medicine, ²Department of Surgery,The University of Maryland, ³Department of Pathology & Immunology,Washington University School of Medicine, ⁴Department of Medicine,Washington University School of Medicine

Journal (Immunology and Infection)

Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs

Fernando Sanchez-Valdez¹, Ángel M. Padilla¹, Juan M. Bustamante¹, Caleb W. D. Hawkins¹, Rick L. Tarleton^1,2

¹Center for Tropical and Emerging Global Diseases,University of Georgia, ²Department of Cellular Biology,University of Georgia

Journal (Immunology and Infection)

Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms

Pranav S. J. B. Rana¹, Erin S. Gloag², Daniel J. Wozniak^1,2

¹Department of Microbiology,The Ohio State University, ²Department of Microbial Infection and Immunity,The Ohio State University

Journal (Immunology and Infection)

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Xavier Cabana-Puig¹, Xin M. Luo¹

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Journal (Immunology and Infection)

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome

Sarah de Oliveira Rodrigues*^1,2,5, Matheus Augusto Patricio de Almeida*^1,2,6, Hugo Caire Castro-Faria-Neto^1,3,4, Adriana Ribeiro Silva^1,3, Cassiano Felippe Gonçalves-de-Albuquerque^1,2,4,5,6

¹Laboratório de Imunofarmacologia,Fundação Oswaldo Cruz, FIOCRUZ, ²Laboratório de Imunofarmacologia, Departamento de Bioquímica, Instituto Biomédico,Universidade Federal do Estado do Rio de Janeiro, ³Programa de Pós-Graduação em Biologia Celular e Molecular,Instituto Oswaldo Cruz, FIOCRUZ, ⁴Programa de Pós-Graduação em Biologia Molecular e Celular,Universidade Federal do Estado do Rio de Janeiro, ⁵Programa de Pós-Graduação em Ciências e Biotecnologia,Universidade Federal Fluminense, ⁶Programa de Pós-Graduação em Neurociências,Universidade Federal Fluminense

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