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JOVE JOURNAL
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Journal (Developmental Biology)
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
Poon Yu Ching
,
Clement Wang
,
Su Hang
,
Pentao Liu
3
,
Ryohichi Sugimura
3
1
Major in Molecular Biology and Biotechnology, School of Biological Sciences
,
The University of Hong Kong
,
2
School of Biomedical Sciences
,
The University of Hong Kong
,
3
Centre for Translational Stem Cell Biology
Author
Poon Yu Ching
,
Clement Wang
,
Su Hang
,
Pentao Liu
3
,
Ryohichi Sugimura
3
Institution
1
Major in Molecular Biology and Biotechnology, School of Biological Sciences
,
The University of Hong Kong
,
2
School of Biomedical Sciences
,
The University of Hong Kong
,
3
Centre for Translational Stem Cell Biology
AUTHORS
Journal (Developmental Biology)
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
Carla Guzman-Moreno*
1
,
Peizhao Zhang*
2
,
Olivia R. Phillips*
1
,
Mathias Block*
3
,
Benjamin J. Glennon*
1
,
Meghan Holbrook
,
Lauren Weigand
,
Cecilia W. Lo
,
Jiuann-Huey I. Lin
4
1
Department of Developmental Biology
,
University of Pittsburgh
,
2
Department of Biological Sciences
,
University of Pittsburgh
,
3
Département de Biologie
,
École Normale Supérieure de Lyon
,
4
Department of Critical Care Medicine
,
University of Pittsburgh
Author
Carla Guzman-Moreno*
1
,
Peizhao Zhang*
2
,
Olivia R. Phillips*
1
,
Mathias Block*
3
,
Benjamin J. Glennon*
1
,
Meghan Holbrook
,
Lauren Weigand
,
Cecilia W. Lo
,
Jiuann-Huey I. Lin
4
Institution
1
Department of Developmental Biology
,
University of Pittsburgh
,
2
Department of Biological Sciences
,
University of Pittsburgh
,
3
Département de Biologie
,
École Normale Supérieure de Lyon
,
4
Department of Critical Care Medicine
,
University of Pittsburgh
AUTHORS
Journal (Developmental Biology)
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
Chantal K. Diallo
,
Andrew J. Modzelewski
1
Department of Biomedical Sciences, School of Veterinary Medicine
,
The University of Pennsylvania
Author
Chantal K. Diallo
,
Andrew J. Modzelewski
Institution
1
Department of Biomedical Sciences, School of Veterinary Medicine
,
The University of Pennsylvania
AUTHORS
Journal (Developmental Biology)
Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells
Sudipta Mahato*
1,2
,
Trupti Agrawal*
1,2
,
Divya Pidishetty*
1,2
,
Savitri Maddileti
,
Vinay Kumar Pulimamidi
3
,
Indumathi Mariappan
1
Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre
,
LV Prasad Eye Institute
,
2
Manipal Academy of Higher Education
,
3
Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School
Author
Sudipta Mahato*
1,2
,
Trupti Agrawal*
1,2
,
Divya Pidishetty*
1,2
,
Savitri Maddileti
,
Vinay Kumar Pulimamidi
3
,
Indumathi Mariappan
Institution
1
Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre
,
LV Prasad Eye Institute
,
2
Manipal Academy of Higher Education
,
3
Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School
AUTHORS
Journal (Developmental Biology)
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
John R. Klem
,
Raèden Gray
,
C. Ben Lovely
1
Alcohol Research Center, Department of Biochemistry and Molecular Genetics
,
University of Louisville
Author
John R. Klem
,
Raèden Gray
,
C. Ben Lovely
Institution
1
Alcohol Research Center, Department of Biochemistry and Molecular Genetics
,
University of Louisville
AUTHORS
Journal (Developmental Biology)
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
Daniela Londoño-Vásquez
2
,
Alexander Jurkevich
,
Ahmed Z. Balboula
1
Animal Sciences Research Center
,
University of Missouri
,
2
Universidad Nacional de Colombia
,
3
Advanced Light Microscopy Core
,
University of Missouri
Author
Daniela Londoño-Vásquez
2
,
Alexander Jurkevich
,
Ahmed Z. Balboula
Institution
1
Animal Sciences Research Center
,
University of Missouri
,
2
Universidad Nacional de Colombia
,
3
Advanced Light Microscopy Core
,
University of Missouri
AUTHORS
Journal (Developmental Biology)
Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
Aishwarya Swaminathan
,
Yung Hwang
,
Ashley Winward
,
Merav Socolovsky
1
Department of Molecular, Cell and Cancer Biology
,
UMASS Chan Medical School
Author
Aishwarya Swaminathan
,
Yung Hwang
,
Ashley Winward
,
Merav Socolovsky
Institution
1
Department of Molecular, Cell and Cancer Biology
,
UMASS Chan Medical School
AUTHORS
Journal (Developmental Biology)
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
Mansour Aboelenain
2,3
,
Karen Schindler
2
,
Cecilia S. Blengini
2
1
Department of Genetics
,
Rutgers, The State University of New Jersey
,
2
Human Genetics Institute of New Jersey
,
3
Department of Theriogenology, Faculty of Veterinary Medicine
,
Mansoura University
Author
Mansour Aboelenain
2,3
,
Karen Schindler
2
,
Cecilia S. Blengini
2
Institution
1
Department of Genetics
,
Rutgers, The State University of New Jersey
,
2
Human Genetics Institute of New Jersey
,
3
Department of Theriogenology, Faculty of Veterinary Medicine
,
Mansoura University
AUTHORS
Journal (Developmental Biology)
An Efficient Clearing Protocol for the Study of Seed Development in Tomato (
Solanum lycopersicum
L.)
Yixuan Feng
2
,
Tai Wang
2,3
,
Lingtong Liu
1
Key Laboratory of Plant Molecular Physiology, Institute of Botany
,
Chinese Academy of Sciences
,
2
College of Life Science
,
University of Chinese Academy of Sciences
,
3
Innovative Academy of Seed Design
,
Chinese Academy of Sciences
Author
Yixuan Feng
2
,
Tai Wang
2,3
,
Lingtong Liu
Institution
1
Key Laboratory of Plant Molecular Physiology, Institute of Botany
,
Chinese Academy of Sciences
,
2
College of Life Science
,
University of Chinese Academy of Sciences
,
3
Innovative Academy of Seed Design
,
Chinese Academy of Sciences
AUTHORS
Journal (Developmental Biology)
Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage
Jayesh Kumar Sevak
,
Suchitra D. Gopinath
1
Translational Health Science and Technology Institute (THSTI)
,
NCR Biotech Science Cluster
Author
Jayesh Kumar Sevak
,
Suchitra D. Gopinath
Institution
1
Translational Health Science and Technology Institute (THSTI)
,
NCR Biotech Science Cluster
AUTHORS
Journal (Developmental Biology)
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Dina H. Kassem
,
Sarah A. Habib
,
Omar I. Badr
,
Mohamed M. Kamal
2,3
1
Biochemistry Department, Faculty of Pharmacy
,
Ain Shams University
,
2
Pharmacology and Biochemistry Department, Faculty of Pharmacy
,
The British University in Egypt
,
3
Center for Drug Research and Development (CDRD), Faculty of Pharmacy
,
The British University in Egypt
Author
Dina H. Kassem
,
Sarah A. Habib
,
Omar I. Badr
,
Mohamed M. Kamal
2,3
Institution
1
Biochemistry Department, Faculty of Pharmacy
,
Ain Shams University
,
2
Pharmacology and Biochemistry Department, Faculty of Pharmacy
,
The British University in Egypt
,
3
Center for Drug Research and Development (CDRD), Faculty of Pharmacy
,
The British University in Egypt
AUTHORS
Journal (Developmental Biology)
CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
Manabu Ozawa*
1
,
Chihiro Emori*
2
,
Masahito Ikawa
2
1
Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science
,
The University of Tokyo
,
2
Research Institute for Microbial Diseases
,
Osaka University
Author
Manabu Ozawa*
1
,
Chihiro Emori*
2
,
Masahito Ikawa
2
Institution
1
Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science
,
The University of Tokyo
,
2
Research Institute for Microbial Diseases
,
Osaka University
AUTHORS
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