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Gene Expression Analyses in Human Follicles
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Developmental Biology
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JoVE Journal Developmental Biology
Gene Expression Analyses in Human Follicles

Gene Expression Analyses in Human Follicles

DOI:
10.3791/64807-v

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09:09 min

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February 17, 2023

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, ,
1Research Laboratory on Human Reproduction,Université libre de Bruxelles (ULB), 2Fertility Clinic, Erasme Hospital,Université libre de Bruxelles (ULB)

Chapters

  • 00:05Introduction
  • 00:54Thawing of Cryopreserved Ovarian Tissue
  • 01:58Follicle Isolation
  • 04:26RNA Extraction
  • 07:01Results: Isolation and Gene Expression Analyses in Human Ovarian Follicles
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol outlining how to isolate human ovarian follicles from frozen-thawed cortical tissue to perform gene expression analyses.

Tags

Gene ExpressionOvarian FolliclesGerm CellsGene Expression AnalysisFollicle IsolationEnzymatic IsolationMechanical IsolationOvarian Cortical FragmentCryoprotectant SolutionLeibovitz 15 MediumDissection MediumDigestion MediumScalpelPetri DishLiquid NitrogenRTCarbon DioxideIncubator

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