JOVE JOURNAL

Biology

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Journal (Biology)

Strategies to Enhance Cultivation of Anaerobic Bacteria from Gastrointestinal Tract of Chicken

Harshita Naithani², Bibiana Rios-Galicia², Amélia Camarinha Silva², Jana Seifert²

¹Institute of Animal Science,University of Hohenheim, ²HoLMiR - Hohenheim Center for Livestock Microbiome Research,University of Hohenheim

Journal (Biology)

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Changyue Liu*^1,2, Zhengyang Lei*^1,2, Lijin Lian, Likun Zhang², Zhicheng Du², Peiwu Qin²

¹Center of Precision Medicine and Healthcare,Tsinghua-Berkeley Shenzhen Institute, ²Institute of Biopharmaceutics and Health Engineering,Tsinghua Shenzhen International Graduate School

Journal (Biology)

Treatment of THP-1 Cells with LPS/ATP for Deciphering Cell Death Mechanisms

Huan Wang, Yuejia Lan, Cuiping Chen, Lu Yang, Jiayi Sun, Yong Zeng, Jiasi Wu

¹State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine, ²Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, ³Acupuncture and Tuina School,Chengdu University of Traditional Chinese Medicine

Journal (Biology)

LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation

Huan Wang, Yuejia Lan, Cuiping Chen, Lu Yang, Jiayi Sun, Yong Zeng, Jiasi Wu

¹State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine, ²Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, ³Acupuncture and Tuina School,Chengdu University of Traditional Chinese Medicine

Journal (Biology)

Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing

Line O. Elingaard-Larsen², Katie L. Whytock, Adeline Divoux, Meghan Hopf, Erin E. Kershaw, Jamie N. Justice, Bret H. Goodpaster, Nancy E. Lane, Lauren M. Sparks

¹Translational Research Institute,AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism,University of Pittsburgh, ⁴Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health

Journal (Biology)

Pulverization and Homogenization of Frozen Human Intermuscular Adipose Tissue Samples for Nuclei Isolation

Line O. Elingaard-Larsen², Katie L. Whytock, Adeline Divoux, Meghan Hopf, Erin E. Kershaw, Jamie N. Justice, Bret H. Goodpaster, Nancy E. Lane, Lauren M. Sparks

¹Translational Research Institute,AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism,University of Pittsburgh, ⁴Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health

Journal (Biology)

Single-Cell Nuclei Isolation from Homogenized Human Intermuscular Adipose Tissue Samples for RNA Sequencing

Line O. Elingaard-Larsen², Katie L. Whytock, Adeline Divoux, Meghan Hopf, Erin E. Kershaw, Jamie N. Justice, Bret H. Goodpaster, Nancy E. Lane, Lauren M. Sparks

¹Translational Research Institute,AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism,University of Pittsburgh, ⁴Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health

Journal (Biology)

Digestion of Mammary Tissue for Isolating Human Mammary Epithelial Cells

Linfeng Qian*¹, Jiaxi Yan*¹, Shiqi Chen, Maryam Mohanmmed Abbas Karekad, Yue Wu⁴, Xunwei Wu, Xiaohong Xu⁴, Xiaoqun Zhang⁷

¹The First Clinical Medical College of Zhejiang Chinese Medical University, ²The International Education College of Zhejiang Chinese Medical University, ³Department of Breast Surgery,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁴Bozhou District Hospital of Traditional Chinese Medicine, ⁵Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School,Hangzhou Medical College, ⁶Department of Surgery, Kaihua District,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁷Kaihua County Hospital of Traditional Chinese Medicine

Journal (Biology)

Isolation and Culture of Primary Human Mammary Epithelial Cells

Linfeng Qian*¹, Jiaxi Yan*¹, Shiqi Chen, Maryam Mohanmmed Abbas Karekad, Yue Wu⁴, Xunwei Wu, Xiaohong Xu⁴, Xiaoqun Zhang⁷

¹The First Clinical Medical College of Zhejiang Chinese Medical University, ²The International Education College of Zhejiang Chinese Medical University, ³Department of Breast Surgery,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁴Bozhou District Hospital of Traditional Chinese Medicine, ⁵Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School,Hangzhou Medical College, ⁶Department of Surgery, Kaihua District,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁷Kaihua County Hospital of Traditional Chinese Medicine

Journal (Biology)

Isolation of Primary Porcine Retinal Pigment Epithelial Cells for In Vitro Modeling

Chase A. Paterson, Dillon Weatherston, Teren Teeples, Elizabeth Vargis

¹Department of Biological Engineering,Utah State University

Journal (Biology)

Porcine Eye Dissection to Isolate the Retinal Pigment Epithelium (RPE) Cells

Chase A. Paterson, Dillon Weatherston, Teren Teeples, Elizabeth Vargis

¹Department of Biological Engineering,Utah State University

Journal (Biology)

Neural Retina Removal and Retinal Pigment Epithelium (RPE) Cell Isolation from Porcine Eyes for In Vitro Retinal Research

Chase A. Paterson, Dillon Weatherston, Teren Teeples, Elizabeth Vargis

¹Department of Biological Engineering,Utah State University

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