JOVE JOURNAL

Biologie

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Journal (Biology)

Culture and Transfection of Naegleria gruberi Trophozoites for Molecular Studies

Brian T. Nguyen¹, Nora M. Chapman², John C. Mullican³, Kristen M. Drescher¹

¹Department of Medical Microbiology and Immunology,Creighton University School of Medicine, ²Department of Pathology and Microbiology,University of Nebraska Medical Center, ³Department of Biology,Washburn University

Journal (Biology)

Closed Circular Extrachromosomal Ribosomal DNA (rDNA) Containing Element (CERE) Isolation from Transfected Naegleria Gruberi Trophozoites and PCR Analysis

Brian T. Nguyen¹, Nora M. Chapman², John C. Mullican³, Kristen M. Drescher¹

¹Department of Medical Microbiology and Immunology,Creighton University School of Medicine, ²Department of Pathology and Microbiology,University of Nebraska Medical Center, ³Department of Biology,Washburn University

Journal (Biology)

Optimizing Mouse Primary Lens Epithelial Cell Culture: A Comprehensive Guide to Trypsinization

Yu Yu*¹, Jinmin Zhang*¹, Hongli Wu^1,2

¹Pharmaceutical Sciences, College of Pharmacy,University of North Texas Health Science Center, ²North Texas Eye Research Institute,University of North Texas Health Science Center

Journal (Biology)

Isolating Mouse Lens Epithelial Cells to Study Lens-Related Disorders

Yu Yu*¹, Jinmin Zhang*¹, Hongli Wu^1,2

¹Pharmaceutical Sciences, College of Pharmacy,University of North Texas Health Science Center, ²North Texas Eye Research Institute,University of North Texas Health Science Center

Journal (Biology)

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius

Zahraa Al-Baqsami*^1,2,3, Rebecca Lowry Palmer*^1,3, Gwyneth Darwent¹, Andrew J. McBain², Christopher G. Knight³, Danna R. Gifford¹

¹Division of Evolution, Infection and Genomics, School of Biological Sciences,The University of Manchester, ²Division of Pharmacy and Optometry, School of Health Sciences,The University of Manchester, ³Department of Earth and Environmental Sciences, School of Natural Sciences,The University of Manchester

Journal (Biology)

Reviving Sulfolobus acidocaldarius Cultures for Temperature Evolution Experimentation

Zahraa Al-Baqsami*^1,2,3, Rebecca Lowry Palmer*^1,3, Gwyneth Darwent¹, Andrew J. McBain², Christopher G. Knight³, Danna R. Gifford¹

¹Division of Evolution, Infection and Genomics, School of Biological Sciences,The University of Manchester, ²Division of Pharmacy and Optometry, School of Health Sciences,The University of Manchester, ³Department of Earth and Environmental Sciences, School of Natural Sciences,The University of Manchester

Journal (Biology)

Initiation of Independent Lineages of Sulfolobus acidocaldarius Cultures for Temperature Evolution Experimentation

Zahraa Al-Baqsami*^1,2,3, Rebecca Lowry Palmer*^1,3, Gwyneth Darwent¹, Andrew J. McBain², Christopher G. Knight³, Danna R. Gifford¹

¹Division of Evolution, Infection and Genomics, School of Biological Sciences,The University of Manchester, ²Division of Pharmacy and Optometry, School of Health Sciences,The University of Manchester, ³Department of Earth and Environmental Sciences, School of Natural Sciences,The University of Manchester

Journal (Biology)

Temperature Evolution Experimentation Using Sulfolobus acidocldarius

Zahraa Al-Baqsami*^1,2,3, Rebecca Lowry Palmer*^1,3, Gwyneth Darwent¹, Andrew J. McBain², Christopher G. Knight³, Danna R. Gifford¹

¹Division of Evolution, Infection and Genomics, School of Biological Sciences,The University of Manchester, ²Division of Pharmacy and Optometry, School of Health Sciences,The University of Manchester, ³Department of Earth and Environmental Sciences, School of Natural Sciences,The University of Manchester

Journal (Biology)

Post-Evolution Growth Assays of Sulfolobus acidocaldarius Ancestral and Evolved Lineages

Zahraa Al-Baqsami*^1,2,3, Rebecca Lowry Palmer*^1,3, Gwyneth Darwent¹, Andrew J. McBain², Christopher G. Knight³, Danna R. Gifford¹

¹Division of Evolution, Infection and Genomics, School of Biological Sciences,The University of Manchester, ²Division of Pharmacy and Optometry, School of Health Sciences,The University of Manchester, ³Department of Earth and Environmental Sciences, School of Natural Sciences,The University of Manchester

Journal (Biology)

Amplification of Leptospira DNA and its Transfer on the Membrane Using a Dot-Blot Apparatus

Eduardo Delgadillo-Tellez¹, Beatriz Zavaleta-Villa¹, Daniel Atilano-López², Rigoberto Hernández-Castro³, Angélica Olivo-Díaz¹, Erika Margarita Carrillo-Casas¹

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Journal (Biology)

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples

Eduardo Delgadillo-Tellez¹, Beatriz Zavaleta-Villa¹, Daniel Atilano-López², Rigoberto Hernández-Castro³, Angélica Olivo-Díaz¹, Erika Margarita Carrillo-Casas¹

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Journal (Biology)

DNA-Membrane Fixation and Probe Hybridisation for Leptospira Detection in Water Samples

Eduardo Delgadillo-Tellez¹, Beatriz Zavaleta-Villa¹, Daniel Atilano-López², Rigoberto Hernández-Castro³, Angélica Olivo-Díaz¹, Erika Margarita Carrillo-Casas¹

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

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