JOVE JOURNAL

Biology

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Journal (Biology)

Anaerobic Bacterial Isolation from Chicken Digesta as a Cultivation Improvement

Harshita Naithani^1,2, Bibiana Rios-Galicia^1,2, Amélia Camarinha Silva^1,2, Jana Seifert^1,2

¹Institute of Animal Science,University of Hohenheim, ²HoLMiR - Hohenheim Center for Livestock Microbiome Research,University of Hohenheim

Journal (Biology)

Strategies to Enhance Cultivation of Anaerobic Bacteria from Gastrointestinal Tract of Chicken

Harshita Naithani^1,2, Bibiana Rios-Galicia^1,2, Amélia Camarinha Silva^1,2, Jana Seifert^1,2

¹Institute of Animal Science,University of Hohenheim, ²HoLMiR - Hohenheim Center for Livestock Microbiome Research,University of Hohenheim

Journal (Biology)

Targeted Isolation of Specific Myo-Inositol Utilizing Bacterial Groups from Chicken Digesta

Harshita Naithani^1,2, Bibiana Rios-Galicia^1,2, Amélia Camarinha Silva^1,2, Jana Seifert^1,2

¹Institute of Animal Science,University of Hohenheim, ²HoLMiR - Hohenheim Center for Livestock Microbiome Research,University of Hohenheim

Journal (Biology)

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Changyue Liu*^1,2, Zhengyang Lei*^1,2, Lijin Lian², Likun Zhang^1,2, Zhicheng Du^1,2, Peiwu Qin^1,2

¹Center of Precision Medicine and Healthcare,Tsinghua-Berkeley Shenzhen Institute, ²Institute of Biopharmaceutics and Health Engineering,Tsinghua Shenzhen International Graduate School

Journal (Biology)

Treatment of THP-1 Cells with LPS/ATP for Deciphering Cell Death Mechanisms

Huan Wang¹, Yuejia Lan¹, Cuiping Chen¹, Lu Yang², Jiayi Sun², Yong Zeng¹, Jiasi Wu³

¹State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine, ²Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, ³Acupuncture and Tuina School,Chengdu University of Traditional Chinese Medicine

Journal (Biology)

LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation

Huan Wang¹, Yuejia Lan¹, Cuiping Chen¹, Lu Yang², Jiayi Sun², Yong Zeng¹, Jiasi Wu³

¹State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine, ²Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, ³Acupuncture and Tuina School,Chengdu University of Traditional Chinese Medicine

Journal (Biology)

Pulverization and Homogenization of Frozen Human Intermuscular Adipose Tissue Samples for Nuclei Isolation

Line O. Elingaard-Larsen^1,2, Katie L. Whytock¹, Adeline Divoux¹, Meghan Hopf¹, Erin E. Kershaw³, Jamie N. Justice⁴, Bret H. Goodpaster¹, Nancy E. Lane⁵, Lauren M. Sparks¹

¹Translational Research Institute,AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism,University of Pittsburgh, ⁴Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health

Journal (Biology)

Single-Cell Nuclei Isolation from Homogenized Human Intermuscular Adipose Tissue Samples for RNA Sequencing

Line O. Elingaard-Larsen^1,2, Katie L. Whytock¹, Adeline Divoux¹, Meghan Hopf¹, Erin E. Kershaw³, Jamie N. Justice⁴, Bret H. Goodpaster¹, Nancy E. Lane⁵, Lauren M. Sparks¹

¹Translational Research Institute,AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism,University of Pittsburgh, ⁴Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health

Journal (Biology)

Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing

Line O. Elingaard-Larsen^1,2, Katie L. Whytock¹, Adeline Divoux¹, Meghan Hopf¹, Erin E. Kershaw³, Jamie N. Justice⁴, Bret H. Goodpaster¹, Nancy E. Lane⁵, Lauren M. Sparks¹

¹Translational Research Institute,AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism,University of Pittsburgh, ⁴Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health

Journal (Biology)

Isolation and Culture of Primary Human Mammary Epithelial Cells

Linfeng Qian*¹, Jiaxi Yan*¹, Shiqi Chen¹, Maryam Mohanmmed Abbas Karekad², Yue Wu^3,4, Xunwei Wu⁵, Xiaohong Xu^3,4, Xiaoqun Zhang^6,7

¹The First Clinical Medical College of Zhejiang Chinese Medical University, ²The International Education College of Zhejiang Chinese Medical University, ³Department of Breast Surgery,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁴Bozhou District Hospital of Traditional Chinese Medicine, ⁵Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School,Hangzhou Medical College, ⁶Department of Surgery, Kaihua District,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁷Kaihua County Hospital of Traditional Chinese Medicine

Journal (Biology)

Digestion of Mammary Tissue for Isolating Human Mammary Epithelial Cells

Linfeng Qian*¹, Jiaxi Yan*¹, Shiqi Chen¹, Maryam Mohanmmed Abbas Karekad², Yue Wu^3,4, Xunwei Wu⁵, Xiaohong Xu^3,4, Xiaoqun Zhang^6,7

¹The First Clinical Medical College of Zhejiang Chinese Medical University, ²The International Education College of Zhejiang Chinese Medical University, ³Department of Breast Surgery,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁴Bozhou District Hospital of Traditional Chinese Medicine, ⁵Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School,Hangzhou Medical College, ⁶Department of Surgery, Kaihua District,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁷Kaihua County Hospital of Traditional Chinese Medicine

Journal (Biology)

Isolation of Primary Porcine Retinal Pigment Epithelial Cells for In Vitro Modeling

Chase A. Paterson¹, Dillon Weatherston¹, Teren Teeples¹, Elizabeth Vargis¹

¹Department of Biological Engineering,Utah State University

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