JOVE JOURNAL

Biochemistry

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A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Khanit Sa-ngiamsuntorn¹, Piyanoot Thongsri², Yongyut Pewkliang², Suparerk Borwornpinyo^3,4, Adisak Wongkajornsilp⁵

¹Department of Biochemistry, Faculty of Pharmacy,Mahidol University, ²Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital,Mahidol University, ³Excellent Center for Drug Discovery, Faculty of Science,Mahidol University, ⁴Department of Biotechnology, Faculty of Science,Mahidol University, ⁵Department of Pharmacology, Faculty of Medicine Siriraj Hospital,Mahidol University

Journal (Biochemistry)

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Patrícia M. S. dos Passos*¹, Valentine Spagnol*², Camila R.S.T.B de Correia¹, Felipe R. Teixeira^1,2

¹Department of Genetic and Evolution,Federal University of São Carlos, ²Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto,University of São Paulo

Journal (Biochemistry)

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Hugo Wioland¹, Foad Ghasemi¹, Jahnavi Chikireddy¹, Guillaume Romet-Lemonne¹, Antoine Jégou¹

¹Université Paris Cité, CNRS,Institut Jacques Monod

Journal (Biochemistry)

Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry

Marcos A. Formiga-Jr¹, Juliana Camacho-Pereira¹

¹Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro

Journal (Biochemistry)

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Dylan Nicholas Tabang¹, Danqing Wang¹, Lingjun Li^1,2

¹Department of Chemistry,University of Wisconsin-Madison, ²School of Pharmacy,University of Wisconsin-Madison

Journal (Biochemistry)

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Yazgan Tuna¹, Amer Al-Hiyasat¹, Jonathon Howard^1,2

¹Department of Molecular Biophysics & Biochemistry,Yale University, ²Department of Physics,Yale University

Journal (Biochemistry)

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Kali A. Smolen¹, Arminja N. Kettenbach^1,2

¹Department of Biochemistry and Cell Biology,Geisel School of Medicine at Dartmouth, ²Norris Cotton Cancer Center

Journal (Biochemistry)

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Kevin Pounot^1,2, Markus Appel², Christian Beck^1,2, Martin Weik³, Giorgio Schirò³, Yann Fichou⁴, Tilo Seydel², Frank Schreiber¹

¹Institut für Angewandte Physik,Universität Tübingen, ²Institut Max von Laue - Paul Langevin (ILL), ³Institut de Biologie Structurale,Université Grenoble Alpes, ⁴Institut Européen de Chimie et Biologie, Bordeaux INP, Chimie et Biologie des Membranes et des Nanoobjets (CBMN),Université de Bordeaux

Journal (Biochemistry)

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Marianna Maniaci*^1,2, Federica Marini*¹, Enrico Massignani^1,2, Tiziana Bonaldi¹

¹Department of Experimental Oncology,European Institute of Oncology IRCCS (IEO), ²European School of Molecular Medicine (SEMM), c/o Campus IFOM-IEO

Journal (Biochemistry)

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Óscar A. Muñoz-Bernal¹, Alma A. Vazquez-Flores¹, Emilio Alvarez-Parrilla¹, Nina R. Martínez-Ruiz¹, Laura A. de la Rosa¹

¹Department of Chemical Biological Sciences,Universidad Autónoma de Ciudad Juárez

Journal (Biochemistry)

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Hui-Lan Chang*¹, Kang-Yi Su*^1,2, Steven D. Goodman³, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Sui-Yuan Chang^1,2, Woei-horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, ²Department of Laboratory Medicine,National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University

Journal (Biochemistry)

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Shawn W. Schowe¹, Conner J. Langeberg², Erich G. Chapman², Kitty Brown³, Marino J. E. Resendiz¹

¹Department of Chemistry,University of Colorado, Denver, ²Department of Chemistry & Biochemistry,University of Denver, ³Analytical Resources Core Bioanalysis & Omics,Colorado State University

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