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JOVE JOURNAL
Biochemistry
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Journal (Biochemistry)
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
Yuno Natsume
,
Hsin-i Wen
,
Tong Zhu
,
Kazumi Itoh
,
Li Sheng
,
Kensuke Kurihara
3,4
1
Department of Mathematical and Physical Sciences, Faculty of Science
,
Japan Women's University
,
2
Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience
,
National Institutes of Natural Sciences
,
3
Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science
,
National Institutes of Natural Sciences
,
4
Research Center for Complex Systems Biology
,
The University of Tokyo
Author
Yuno Natsume
,
Hsin-i Wen
,
Tong Zhu
,
Kazumi Itoh
,
Li Sheng
,
Kensuke Kurihara
3,4
Institution
1
Department of Mathematical and Physical Sciences, Faculty of Science
,
Japan Women's University
,
2
Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience
,
National Institutes of Natural Sciences
,
3
Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science
,
National Institutes of Natural Sciences
,
4
Research Center for Complex Systems Biology
,
The University of Tokyo
AUTHORS
Journal (Biochemistry)
Laboratory Scale Production and Purification of a Therapeutic Antibody
Zehra Elgundi
,
Vicki Sifniotis
,
Mouhamad Reslan
,
Esteban Cruz
,
Veysel Kayser
1
Faculty of Pharmacy
,
University of Sydney
Author
Zehra Elgundi
,
Vicki Sifniotis
,
Mouhamad Reslan
,
Esteban Cruz
,
Veysel Kayser
Institution
1
Faculty of Pharmacy
,
University of Sydney
AUTHORS
Journal (Biochemistry)
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Megumi Tsuchiya*
1
,
M. Rezaul Karim*
2
,
Taro Matsumoto
,
Hidesato Ogawa
,
Hiroaki Taniguchi
4,5
1
Graduate School of Frontier Biosciences
,
Osaka University
,
2
Department of Biotechnology and Genetic Engineering
,
Jahangirnagar University
,
3
Division of Cell Regeneration and Transplantation
,
School of Medicine, Nihon University
,
4
Institute of Genetics and Animal Breeding of the Polish Academy of Sciences
,
5
Graduate School of Life and Medical Sciences
,
Doshisha University
Author
Megumi Tsuchiya*
1
,
M. Rezaul Karim*
2
,
Taro Matsumoto
,
Hidesato Ogawa
,
Hiroaki Taniguchi
4,5
Institution
1
Graduate School of Frontier Biosciences
,
Osaka University
,
2
Department of Biotechnology and Genetic Engineering
,
Jahangirnagar University
,
3
Division of Cell Regeneration and Transplantation
,
School of Medicine, Nihon University
,
4
Institute of Genetics and Animal Breeding of the Polish Academy of Sciences
,
5
Graduate School of Life and Medical Sciences
,
Doshisha University
AUTHORS
Journal (Biochemistry)
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and
In Situ
Proximity Ligation Assay
Xinzhou Zhu
,
Andrea Zelmer
,
Sven Wellmann
2
1
University Children's Hospital Basel (UKBB)
,
University of Basel
,
2
Department of Clinical Research
,
University of Basel
Author
Xinzhou Zhu
,
Andrea Zelmer
,
Sven Wellmann
2
Institution
1
University Children's Hospital Basel (UKBB)
,
University of Basel
,
2
Department of Clinical Research
,
University of Basel
AUTHORS
Journal (Biochemistry)
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Carlos Eduardo Espinosa-de la Garza*
1
,
Rodolfo Daniel Salazar-Flores*
1
,
Néstor Octavio Pérez
,
Luis Francisco Flores-Ortiz
,
Emilio Medina-Rivero
1
Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.
Author
Carlos Eduardo Espinosa-de la Garza*
1
,
Rodolfo Daniel Salazar-Flores*
1
,
Néstor Octavio Pérez
,
Luis Francisco Flores-Ortiz
,
Emilio Medina-Rivero
Institution
1
Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.
AUTHORS
Journal (Biochemistry)
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Gatikrushna Singh*
1
,
Sarah M. Fritz*
2
,
Arnaz Ranji
,
Deepali Singh
,
Kathleen Boris-Lawrie
2
1
Department of Veterinary & Biomedical Sciences
,
University of Minnesota
,
2
Department of Veterinary Biosciences
,
Ohio State University
,
3
School of Biotechnology
,
Gautam Buddha University
Author
Gatikrushna Singh*
1
,
Sarah M. Fritz*
2
,
Arnaz Ranji
,
Deepali Singh
,
Kathleen Boris-Lawrie
2
Institution
1
Department of Veterinary & Biomedical Sciences
,
University of Minnesota
,
2
Department of Veterinary Biosciences
,
Ohio State University
,
3
School of Biotechnology
,
Gautam Buddha University
AUTHORS
Journal (Biochemistry)
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
Jonathan Fischer
,
Roger A. Johnson
,
Elizabeth Boon
1
Department of Chemistry
,
Stony Brook University
,
2
Department of Physiology and Biophysics
,
Stony Brook University
Author
Jonathan Fischer
,
Roger A. Johnson
,
Elizabeth Boon
Institution
1
Department of Chemistry
,
Stony Brook University
,
2
Department of Physiology and Biophysics
,
Stony Brook University
AUTHORS
Journal (Biochemistry)
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Masatake Kanai
,
Shoji Mano
2
,
Mikio Nishimura
1
Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity
,
National Institute for Basic Biology
,
2
Department of Basic Biology
,
SOKENDAI (The Graduate University for Advanced Studies)
,
3
Department of Cell Biology
,
National Institute for Basic Biology
Author
Masatake Kanai
,
Shoji Mano
2
,
Mikio Nishimura
Institution
1
Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity
,
National Institute for Basic Biology
,
2
Department of Basic Biology
,
SOKENDAI (The Graduate University for Advanced Studies)
,
3
Department of Cell Biology
,
National Institute for Basic Biology
AUTHORS
Journal (Biochemistry)
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions
In Vitro
Ryan W. Clinton
,
Jason A. Mears
1
Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology
,
Case Western Reserve University School of Medicine
Author
Ryan W. Clinton
,
Jason A. Mears
Institution
1
Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology
,
Case Western Reserve University School of Medicine
AUTHORS
Journal (Biochemistry)
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Clemens Entzian
,
Thomas Schubert
1
2bind GmbH
Author
Clemens Entzian
,
Thomas Schubert
Institution
1
2bind GmbH
AUTHORS
Journal (Biochemistry)
A Rapid Laser Probing Method Facilitates the Non-invasive and Contact-free Determination of Leaf Thermal Properties
Johannes F. Buyel
2
,
Hannah M. Gruchow
,
Martin Wehner
1
Fraunhofer Institute for Molecular Biology and Applied Ecology IME
,
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.
,
2
Institute for Molecular Biotechnology
,
RWTH Aachen University
,
3
Fraunhofer Institute for Laser Technology ILT
,
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.
Author
Johannes F. Buyel
2
,
Hannah M. Gruchow
,
Martin Wehner
Institution
1
Fraunhofer Institute for Molecular Biology and Applied Ecology IME
,
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.
,
2
Institute for Molecular Biotechnology
,
RWTH Aachen University
,
3
Fraunhofer Institute for Laser Technology ILT
,
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.
AUTHORS
Journal (Biochemistry)
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
Jakob V. Larsen
,
Claus M. Petersen
1
Department of Biomedicine
,
Aarhus University
Author
Jakob V. Larsen
,
Claus M. Petersen
Institution
1
Department of Biomedicine
,
Aarhus University
AUTHORS
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