JOVE JOURNAL

Biochemistry

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Journal (Biochemistry)

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Eliza L. Lewis¹, Aaron M. Leconte¹

¹Department of Chemistry,W.M. Keck Science Department of Claremont Mckenna, Pitzer, and Scripps College

Journal (Biochemistry)

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Simone Culurgioni^1,2, Minzhe Tang^1,2, David R. Hall¹, Martin A. Walsh^1,2

¹Diamond Light Source,Harwell Science & Innovation Campus, ²Research Complex at Harwell,Harwell Science & Innovation Campus

Journal (Biochemistry)

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Kan Xiong^1,2, Paul C. Blainey^1,2

¹Broad Institute,Massachusetts Institute of Technology and Harvard Medical School, ²Dept. of Biological Engineering,Massachusetts Institute of Technology

Journal (Biochemistry)

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Robert Warneford-Thomson^1,4, Chongsheng He^1,2, Simone Sidoli^1,3, Benjamin A. Garcia^1,3, Roberto Bonasio^1,2

¹Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, ²Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, ³Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, ⁴Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine

Journal (Biochemistry)

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Huijuan You*¹, Shimin Le*^2,3, Hu Chen^2,4, Linyan Qin¹, Jie Yan^2,4,5

¹School of Pharmacy, Tongji Medical College,Huazhong University of Science and Technology, ²Mechanobiology Institute,National University of Singapore, ³Department of Physics,National University of Singapore, ⁴Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics,Xiamen University, ⁵Center for Bioimaging Sciences,National University of Singapore

Journal (Biochemistry)

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Antoine Loquet¹, James Tolchard¹, Melanie Berbon¹, Denis Martinez¹, Birgit Habenstein¹

¹Institute of Chemistry, Biology of Membranes, Nanoobjects,UMR5248 CNRS, Université de Bordeaux

Journal (Biochemistry)

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Pan Liu¹, Tomokazu Souma¹, Andrew Zu-Sern Wei¹, Xueying Xie³, Xunrong Luo², Jing Jin¹

¹Division of Nephrology and Hypertension, and the Center for Kidney Research and Therapeutics at the Feinberg Cardiovascular Research Institute,Northwestern University Feinberg School of Medicine, ²Surgery-Organ Transplantation,Northwestern University Feinberg School of Medicine, ³School of Biological Sciences and Medical Engineering,Southeast University

Journal (Biochemistry)

Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy

Yulong Sun¹, Philbert Ip², Avijit Chakrabartty^1,2

¹Department of Medical Biophysics,University of Toronto, ²Department of Biochemistry,University of Toronto

Journal (Biochemistry)

In Vitro Polymerization of F-actin on Early Endosomes

Olivia Muriel^1,2, Cameron Christopher Scott¹, Jorge Larios¹, Vicent Mercier¹, Jean Gruenberg¹

¹Department of Biochemistry,University of Geneva, ²Department of Fundamental Microbiology,University of Lausanne

Journal (Biochemistry)

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Thomas Søndergaard Stenum¹, Michael A. Sørensen¹, Sine Lo Svenningsen¹

¹Department of Biology,University of Copenhagen

Journal (Biochemistry)

Quantitative Immunofluorescence to Measure Global Localized Translation

Jonathan Bergeman¹, Marc-Étienne Huot^1,2

¹Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie,Université Laval, ²CRCHU de Québec: L’Hôtel-Dieu de Québec

Journal (Biochemistry)

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Mo Zhou¹, Mark R. Philips¹

¹Langone Medical Center,New York University

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