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Herstellung von humanen Neurogenin 2-induzierbaren Neuronen in einem dreidimensionalen Suspensionsbioreaktor
This content is Free Access.
1Fraunhofer Project Center for Stem Cell Process Engineering,Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology,Saarland University, 6Facultad de Ciencias del Mar,Universidad Católica del Norte
Capítulos
- 00:04Introduction
- 00:51Precultivation of hiPSCs in the Benchtop Bioreactor System (Day ‐2)
- 02:53Differentiation of hiPSCs into Early Neurons (Day 0)
- 03:29Cryopreservation of Early Neurons (Day 2)
- 05:01Results: Assessing the Generation of hiPSC‐Derived Neurons Using the Benchtop Bioreactor
- 06:40Conclusion
Tags
Keywords: 3D Suspension BioreactorHuman Neurogenin 2-inducible NeuronsInduced Pluripotent Stem CellsIPSC CultureNeuronal DifferentiationHigh-throughput ScreeningRapid And Cost-efficient GenerationCardiomyocytesPre-cultivationTrypsinizationCell SingularizationCell CountingCell SuspensionCentrifugationFeeder-free IPSC Maintenance MediumROCK InhibitorBioreactor Cultivation
