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Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor
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March 17, 2023
DOI:
1Fraunhofer Project Center for Stem Cell Process Engineering,Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology,Saarland University, 6Facultad de Ciencias del Mar,Universidad Católica del Norte
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This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.
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3D Suspension BioreactorHuman Neurogenin 2-inducible NeuronsInduced Pluripotent Stem CellsIPSC CultureNeuronal DifferentiationHigh-throughput ScreeningRapid And Cost-efficient GenerationCardiomyocytesPre-cultivationTrypsinizationCell SingularizationCell CountingCell SuspensionCentrifugationFeeder-free IPSC Maintenance MediumROCK InhibitorBioreactor Cultivation
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Cite this Article
Wihan, J., Karnatz, I., Sébastien, I., Kettenhofen, R., Schmid, B., Clausen, C., Fischer, B., Steeg, R., Zimmermann, H., Neubauer, J. C. Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor. J. Vis. Exp. (193), e65085, doi:10.3791/65085 (2023).
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